HSF1 anticorps (Ser307)

N° du produit ABIN197434

Cet anticorps Lapin Polyclonal détecte spécifiquement HSF1 dans WB et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour HSF1 anticorps (Ser307) (ABIN197434)

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HSF1 anticorps

Épitope: Ser307

Specificité: HSF1 antibody detects endogenous levels of total HSF1 protein.

Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R).

Information d'application

Indications d'application: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry on paraffin sections: 1: 50-1: 100.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur HSF1

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Autre désignation: Heat Shock Factor 1 / HSF1

Sujet: All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38 % sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

ID gène: 3297

NCBI Accession: NP_005517

UniProt: Q00613